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METHOD FOR DETECTING SELF-RENEWALITY OF MOUSE EMBRYONIC STEM CELLS
METHOD FOR DETECTING SELF-RENEWALITY OF MOUSE EMBRYONIC STEM CELLS
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机译:检测小鼠胚胎干细胞自我更新的方法
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摘要
PURPOSE: A method for detecting self-renewality of mouse embryonic stem cells is provided to determine that the mouse embryonic stem cells has self-renewality when one or more genes of a group comprising polynucleotide of sequence number 1 to 3 are overexpressed. CONSTITUTION: A method for detecting self-renewality of mouse embryonic stem cells comprises: a step of preparing nucleic acid sample from mouse embryonic stem cells, and a step of measuring expression amount of genes selected comprising gene of sequence number 1 to 3. The step of measuring expression amount of the gene is performed by through RT-PCR (reverse transcriptase-polymerase chain reaction) or real time-PCR.
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