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METHOD FOR SCREENING INHIBITOR OF CELL CYCLE REGULATOR USING CAENORHABDITIS ELEGANS

机译:利用盲肠念珠菌筛选细胞周期调节因子抑制剂的方法

摘要

A method for screening a cell cycle control factor inhibitor is provided to screen easily drug of bulk in a fast date using Caenorhabditis elegans easy to be observed because a size of intestines nucleus is greater than the nucleus of the other cells. A method for screening a cell cycle control factor inhibitor includes steps of: processing testing material when cultivating Caenorhabditis elegans mutant overexpressing CDC-25 which is a cell cycle control factor; and confirming intestines nucleus number of the Caenorhabditis elegans mutant and confirming CDC-25 deterrent effect. The Caenorhabditis elegans mutant over-expressing the CDC-25 is the Caenorhabditis elegans rr31 mutant.
机译:本发明提供了一种用于筛选细胞周期控制因子抑制剂的方法,以利用易于观察到的秀丽隐杆线虫(Caenorhabditis elegans),快速筛选大量药物,因为肠核的大小大于其他细胞的核。一种筛选细胞周期控制因子抑制剂的方法,包括以下步骤:在培养作为细胞周期控制因子的过量表达秀丽隐杆线虫突变体CDC-25时,处理测试材料;并确定秀丽隐杆线虫突变体的肠核数目,并确认CDC-25的阻遏作用。过量表达CDC-25的秀丽隐杆线虫突变体是秀丽隐杆线虫rr31突变体。

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