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Method for preparation of recombinant protein using RNA binding protein as fusion partner

机译:以RNA结合蛋白为融合伴侣的重组蛋白的制备方法

摘要

The present invention relates to an expression vector containing the gene of the method and the RNA binding protein to produce a recombinant protein with improved water solubility and folding, using the gene of the RNA binding protein as a fusion partner (fusion partner) as a fusion partner, and more particularly Advantageously the fusion partner and for producing an expression vector which connect the gene of the foreign protein, introducing the expression vector into a host cell producing a transformant, and culturing fused to a heterologous protein expressed in a protein form the transformant according to the method of production of recombinant proteins, RNA binding by using the gene of the protein to the fusion partner by expressing RNA- protein complexes in the form of a method for producing recombinant proteins, characterized in that to improve the water-soluble, and the folding of the foreign protein and the RNA binding protein gene relates to expression vectors containing a fusion partner. A method of producing recombinant protein by using the gene of RNA-binding proteins of the present invention as a fusion partner, it is possible to overcome the limitation on the water-soluble and folded in the conventional fusion partners have, widely used for protein drug and industrial protein production It can be.; A fusion partner, RNA-binding proteins
机译:表达载体技术领域本发明涉及一种表达载体,其包含该方法的基因和RNA结合蛋白,以RNA结合蛋白的基因作为融合伴侣(融合伴侣)作为融合蛋白,以生产具有改善的水溶性和折叠性的重组蛋白。伴侣,更特别是融合伴侣,并用于产生连接外源蛋白质基因的表达载体,将表达载体引入产生转化体的宿主细胞中,并与融合于该转化体的蛋白质中表达的异源蛋白质进行培养根据重组蛋白的生产方法,以表达重组蛋白的方法的形式表达RNA-蛋白复合物,利用该蛋白的基因将RNA与融合伴侣结合,其特征在于提高水溶性,以及外源蛋白和RNA结合蛋白基因的折叠涉及含有fus的表达载体离子搭档。通过使用本发明的RNA结合蛋白的基因作为融合伴侣来生产重组蛋白的方法,有可能克服水溶性和折叠在常规的融合伴侣中所具有的局限性,广泛用于蛋白质药物和工业蛋白质的生产都可以。融合伴侣,RNA结合蛋白

著录项

  • 公开/公告号KR100890579B1

    专利类型

  • 公开/公告日2009-04-27

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20020048929

  • 发明设计人 신항철;성백린;최성일;

    申请日2002-08-19

  • 分类号C07K14/00;

  • 国家 KR

  • 入库时间 2022-08-21 19:12:04

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