Method of identification between Hanwoo and foreign beef meats

摘要

in accordance with the present invention and the outpatient beef cattle introduction Wu determine how eseual Y (sex-determining region Y; It made using the RFLP); SRY) gene of restriction fragment length polymorphism (Restriction Fragment Length Polymorphism. As the Y gene is a gene of the boss eseual variety Taurus, the 1707th base is T comprises a single nucleotide polymorphism (SNP), including a variation part in the G nucleotide. Specifically, the introduction of foreign beef and Wu determination method comprising the DNA collected from the beef, and introducing the foreign right, coupling the forward primer and reverse primer and the DNA of each of the collected eseual Y gene, the coupling product and a step of analyzing an amplification step, and then cutting the amplification products polymorphisms in Mse restriction enzymes to amplify PCR introduced a foreign Wu classified genotypes and beef. According to the determination method boss Taurus ( Bos taurus) Origin of Indy boss of hanwooyuk and Caicos derived from foreign It can determine exactly beef introduction (Brahman) in the DNA level.