首页>
外国专利>
METHOD, reagents and KIT FOR DETERMINATION OF RESIDUAL cholesterol-like particles
METHOD, reagents and KIT FOR DETERMINATION OF RESIDUAL cholesterol-like particles
展开▼
机译:测定残留胆固醇样颗粒的方法,试剂和试剂盒
展开▼
页面导航
摘要
著录项
相似文献
摘要
1.method of quantifying cholesterol in the u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 residually like particles in a sample to the aquatic environment, containing the sample and in the presence of (a) (i) u043eu043cu0431u0438u043du0430u0446u0438u0438 one or more types of surfactants selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of: copolymer u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043du0430 and u043fu043eu043bu0438u043eu043au0441u0438u0431u0443u0442u0438u043bu0435u043du0430,u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043d - u0441u0442u0438u0440u043eu043bu0441u043eu0434u0435u0440u0436u0430u0449u0438u0439 u0444u0435u043du0438u043bu043eu0432u044bu0439 ether and u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043d - u043fu043eu043bu0438u043eu043au0441u0438u043fu0440u043eu043fu0438u043bu0435u043du0430u043bu043au0438u043bu043eu0432u044bu0439 ether containing long branched out u0430u043bu043au0438u043bu044cu043du0443u044e group (hereinafter the luggage u043du0430u0447u0430u0435u0442u0441u00a0 as surfactant (d1), and one or more types of surfactants selected from the group of: u0441u043eu0441u0442u043eu00a0u0449u0435u0439 u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043d - u043fu043eu043bu0438u043eu043au0441u0438u043fu0440u043eu043fu0438u043bu0435u043du0430u043bu043au0438u043bu0430u0440u0438u043bu043eu0432u043eu0433u043e ef ira, u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043du0430u043bu043au0438u043bu0430u0440u0438u043bu043eu0432u043eu0433u043e etheru043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043d - u043fu043eu043bu0438u043eu043au0441u0438u043fu0440u043eu043fu0438u043bu0435u043du0430u043bu043au0438u043bu043eu0432u043eu0433u043e ether and u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043d - u043fu043eu043bu0438u043eu043au0441u0438u043fu0440u043eu043fu0438u043bu0435u043du0430u043bu043au0438u043bu043eu0432u043eu0433u043e ether containing a branching unit (u0430u043bu043au0438u043bu044cu043du0443u044e dal (e u043eu0431u043eu0437u043du0430u0447u0430u0435u0442u0441u00a0 as surfactant (d2)), or (ii) the combination of copolymer u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043du0430 and u043fu043eu043bu0438u043eu043au0441u0438u043fu0440u043eu043fu0438u043bu0435u043du0430 (hereinafter referred to as the surfactant u043eu0431u043eu0437u043du0430u0447u0430u0435u0442u0441u00a0 (d3)) and one or two types of u043fu043eu0432u0435u0440u0445u043du043eu0441u0442u043d on the active substances selected from the groupu0441u043eu0441u0442u043eu00a0u0449u0438u0439: u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043du0430u043bu043au0438u043bu0430u0440u0438u043bu043eu0432u043eu0433u043e ether u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043d - u043fu043eu043bu0438u043eu043au0441u0438u043fu0440u043eu043fu0438u043bu0435u043du0430u043bu043au0438u043bu043eu0432u043eu0433u043e ether and u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043d - u043fu043eu043bu0438u043eu043au0441u0438u043fu0440u043eu043fu0438u043bu0435u043du0430u043bu043au0438u043bu043eu0432u043eu0433u043e ether contained with a vast u0430u043bu043au0438u043bu044cu043du0443u044e group (hereinafter referred to as the surfactant u043eu0431u043eu0437u043du0430u0447u0430u0435u0442u0441u00a0 (d4)) and (b) u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0435u0433u043e enzyme u043fu043eu0437u0432u043eu043bu00a0u044eu0449u0438u0439 (c) u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du044du0441u0442u0435u0440u0430u0437u0435 and u0445u043eu043bu0435u0441u0442u0435 u0440u0438u043du043eu043au0441u0438u0434u0430u0437u0435,or (d) in the presence of u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du044du0441u0442u0435u0440u0430u0437u0435 and u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du0434u0435u0433u0438u0434u0440u043eu0433u0435u043du0430u0437u0435 u043eu043au0438u0441u043bu0435u043du043du043eu0439 form u043au043eu0444u0435u0440u043cu0435u043du0442u0430, act on the cholesterol of hrd in the sample with the formation of p u0435u0440u043eu043au0441u0438u0434u0430 hydrogen or restored form u043au043eu0444u0435u0440u043cu0435u043du0442u0430 and u043eu043fu0440u0435u0434u0435u043bu00a0u0442u044c u043eu0431u0440u0430u0437u043eu0432u0430u0432u0448u0438u0435u0441u00a0 hydrogen peroxide or regenerated coenzyme.;2. method for the determination of 1, in which hydrogen peroxide u043fu0440u043eu0432u043eu0434u0438u0442u0441u00a0 by u0432u0437u0430u0438u043cu043eu0434u0435u0439u0441u0442u0432u0438u00a0 formed peroxide hydrogen in the presence of u043fu0435u0440u043eu043au0441u0438u0434u0430u0437u044b with u0445u0440u043eu043cu043eu0433u0435u043du043e m assuming / u0438u0437u043cu0435u043du00a0u044eu0449u0438u043c color during the u043eu043au0438u0441u043bu0435u043du0438u00a0, and the definition of u043eu0431u0440u0430u0437u043eu0432u0430u0432u0448u0435u0433u043eu0441u00a0 u043au0440u0430u0441u0438u0442u0435u043bu00a0.;3. method for 1, in which the definition of the third form u043au043eu0444u0435u0440u043cu0435u043du0442u0430 u043fu0440u043eu0432u043eu0434u0438u0442u0441u00a0 by u0438u0437u043cu0435u0440u0435u043du0438u00a0 u043fu043eu0433u043bu043eu0449u0435u043du0438u00a0 reaction solution.;4. method for 1, in which the definition of u043eu0431u0440u0430u0437u043eu0432u0430u0432u0448u0435u0439u0441u00a0 restored form u043au043eu0444u0435u0440u043cu0435u043du0442u0430 u043fu0440u043eu0432u043eu0434u0438u0442u0441u00a0 by u0432u0437u0430u0438u043cu043eu0434u0435u0439u0441u0442u0432u0438u00a0 u043eu0431u0440u0430u0437u043eu0432u0430u0432u0448u0435u0433u043eu0441u00a0 reduced u043au043eu0444u0435u0440u043cu0435 u043du0442u0430 with u0445u0440u043eu043cu043eu0433u0435u043du043eu043c, assuming / u0438u0437u043cu0435u043du00a0u044eu0449u0438u043c color during the u0432u043eu0441u0441u0442u0430u043du043eu0432u043bu0435u043du0438u00a0 and definition of u043eu0431u0440u0430u0437u043eu0432u0430u0432u0448u0435u0433u043eu0441u00a0 u043au0440u0430u0441u0438u0442u0435u043bu00a0.;5. way on any of the u043fu043f.1 - 4, in which the copolymer u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043du0430 and u043fu043eu043bu0438u043eu043au0441u0438u0431u0443u0442u0435u043bu0435u043du0430 u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a surfactant of general formula (i):;ro (C2H4O) a - (C4H8O) b - (C2H4O) c - h (i);(where a, b and c are the same or different u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u044eu0442 integers from 1 to 200; r is a hydrogen atom or u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 branching or u043du0435u0440u0430u0437u0432u0435u0442u0432u043bu0435u043du043du0443u044e u0430u043bu043au0438u043bu044cu043du0443 yu group).;6. way on any of the u043fu043f.1 - 4, which u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0438u0439 enzyme u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a u0444u043eu0441u0444u043eu043bu0438u043fu0430u0437u0443 d, u0444u043eu0441u0444u043eu043bu0438u043fu0430u0437u0443 with or u0444u043eu0441u0444u043eu043bu0438u043fu0430u0437u0443 a2.;7. reagent u0434u043bu00a0 quantitative u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 cholesterol within it - like particles in a sample containing u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0438u0439 enzyme u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du044du0441u0442u0435u0440u0430 zou, u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du043eu043au0441u0438u0434u0430u0437u0443 and combination of surfactants (d1) and (d2), or a combination of paw paw (d3) and surfactant (d4).;8. reagent on p.7, additionally containing a reagent u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 peroxide of hydrogen.;9. reagent u0434u043bu00a0 quantitative u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 cholesterol within it - like particles in a sample containing u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0438u0439 enzyme u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du044du0441u0442u0435u0440u0430 zou, u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du0434u0435u0433u0438u0434u0440u043eu0433u0435u043du0430u0437u0443, u043eu043au0438u0441u043bu0435u043du043du0443u044e form u043au043eu0444u0435u0440u043cu0435u043du0442u0430 and combination of surfactants (d1) and (d2), or a combination of paw paw (d3) and surfactant (d4).;10. reagent for p.9, additionally containing a reagent u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 restored form u043au043eu0444u0435u0440u043cu0435u043du0442u0430.;11. reagent for any of the u043fu043f.7 - 10, in which the copolymer u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043du0430 and u043fu043eu043bu0438u043eu043au0441u0438u0431u0443u0442u0435u043bu0435u043du0430 u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a surfactant of general formula (i):;ro (C2H4O) a - (C4H8O) b - (C2H4O) c - h (i);(where a, b and c are the same or different u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u044eu0442 integers from 1 to 200; r is a hydrogen atom or u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 branching or u043du0435u0440u0430u0437u0432u0435u0442u0432u043bu0435u043du043du0443u044e u0430u043bu043au0438u043bu044cu043du0443 yu group).;12. reagent for any of the u043fu043f.7 - 10, which u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0438u0439 enzyme u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a u0444u043eu0441u0444u043eu043bu0438u043fu0430u0437u0443 d, u0444u043eu0441u0444u043eu043bu0438u043fu0430u0437u0443 with or u0444u043eu0441u0444u043eu043bu0438u043fu0430u0437u0443 a2.;13. a set of u0434u043bu00a0 quantitative u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 cholesterol within it - like particles in a sample containing a first reagent containing surfactant (d1) and a second reagent, inc u044eu0447u0430u044eu0449u0438u0439 u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0438u0439 enzyme u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du044du0441u0442u0435u0440u0430u0437u0443 and u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du043eu043au0441u0438u0434u0430u0437u0443 and the kit further contains surfactant (d2) at least one per mas u0440u0435u0430u0433u0435u043du0442u0435 or second u0440u0435u0430u0433u0435u043du0442u0435.;14. a set of u0434u043bu00a0 quantitative u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 cholesterol within it - like particles in a sample containing a first reagent containing surfactant (d3), and the second reagent, inc u044eu0447u0430u044eu0449u0438u0439 u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0438u0439 enzyme u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du044du0441u0442u0435u0440u0430u0437u0443 and u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du043eu043au0441u0438u0434u0430u0437u0443 and the kit further contains surfactant (d4) in at least one of the first and second reagents.;15. set on any of the u043fu043f.13 and 14, further containing a reagent u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 hydrogen peroxide in at least one of the first and second reagents.;16.a set of u0434u043bu00a0 quantitative u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 cholesterol within it - like particles in a sample containing a first reagent containing surfactant (d1) and a second reagent, inc u044eu0447u0430u044eu0449u0438u0439 u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0438u0439 enzyme u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du044du0441u0442u0435u0440u0430u0437u0443 and u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du0434u0435u0433u0438u0434u0440u043eu0433u0435u043du0430u0437u0443,with this set contains surfactant (d2) in at least one of the first and second reagents and further comprising u043eu043au0438u0441u043bu0435u043du043du0443u044e form u043au043eu0444u0435u0440u043cu0435u043du0442u0430 at m u0435u0440u0435 in one of the first and second reagents.;17.a set of u0434u043bu00a0 quantitative u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 cholesterol within it - like particles in a sample containing a first reagent containing surfactant (d3), and the second reagent, inc u044eu0447u0430u044eu0449u0438u0439 u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0438u0439 enzyme u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du044du0441u0442u0435u0440u0430u0437u0443 and u0445u043eu043bu0435u0441u0442u0435u0440u0438u043du0434u0435u0433u0438u0434u0440u043eu0433u0435u043du0430u0437u0443,with this set contains surfactant (d4) in at least one of the first and second reagents and further comprising at least u043eu043au0438u0441u043bu0435u043du043du0443u044e form u043au043eu0444u0435u0440u043cu0435u043du0442u0430 in one of the first and second reagents.;18. set on any of the u043fu043f.16 and 17, further containing a reagent u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 restored form u043au043eu0444u0435u0440u043cu0435u043du0442u0430 in at least one of the first and second u0440u0435u0430u0433u0435u043d tov.;19. set on any of the u043fu043f.13 or 16, in which the copolymer u043fu043eu043bu0438u043eu043au0441u0438u044du0442u0438u043bu0435u043du0430 and u043fu043eu043bu0438u043eu043au0441u0438u0431u0443u0442u0435u043bu0435u043du0430 u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a surfactant of general formula (i):;ro (C2H4O) a - (C4H8O) b - (C2H4O) c - h (i);(where a, b and c are the same or different u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u044eu0442 integers from 1 to 200; r is a hydrogen atom or u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 branching or u043du0435u0440u0430u0437u0432u0435u0442u0432u043bu0435u043du043du0443u044e u0430u043bu043au0438u043bu044cu043du0443u044e group).;20. set on any of the u043fu043f.13, 14, 16 and 17, which u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434 - u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0449u0438u0439 enzyme u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a u0444u043eu0441u0444u043eu043bu0438u043fu0430u0437u0443 d, u0444u043eu0441u0444u043eu043bu0438u043fu0430u0437u0443 with or u0444u043eu0441u0444u043eu043bu0438u043fu0430u0437u0443 a2.
展开▼