METHOD OF POULTRY INTESTINE EXAMINATION FOR AMYLOLYTIC ENZYME MICROFLORA ACTIVITY

摘要

FIELD: medicine; veterinary science.;SUBSTANCE: content 1 g of large intestine caecum is sampled. Distilled water 9 ml is added, carefully mixed, centrifuged within 3 min at 4000 rpm. Centrifugate 1 ml is mixed with nutrient mixture containing NaCl 0.9 g, NaHCO₃ 0.3 g. Then starch and distilled water are added. Reactants are the same to be added both in experiment and control test tubes, except that experiment test tubes are incubated at temperature 41°C within 35 minutes, while control test tubes are not incubated. To drop the reaction, hydrochloric acid is added immediately therein. The solutions are colorimetered to evaluate the optical density of solutions and calculate amount of split starch in percentage, value of which is used to estimate amylolytic enzyme activity.;EFFECT: simplified and reduced examination process.;1 ex