RECOMBINANT PLASMID DNA pBMC-RT(A)-hum FOR EXPRESSING PROTEIN OF REVERSE TRANSCRIPTASE OF HUMAN IMMUNODEFICIENCY VIRUS

摘要

FIELD: biology.;SUBSTANCE: present invention relates to biotechnology, particularly to genetic engineering and can be used in the biomedical industry. Proposed is a recombinant plasmid pBMC-RT(A)-hum, meant for expressing reverse transcriptase of the human immunodeficiency virus, and is distinguished by that, it contains an artificial gene (RT(A)-hum) synthesised by an enzyme, which is characterised by a sequence of nucleotides, optimally adapted to expression in cells of mammals.;EFFECT: use of the recombinant plasmid provides for significant increase (6-8 times) in output of the target protein compared with the primary standard.;4 dwg, 1 tbl, 3 ex