CYTOBIOCHEMICAL METHOD OF DETERMINING ACTIVITY OF SUCCINATE DEHYDROGENASE, ENDOGENIC SUCCINIC ACID OXIDATION, SIGNAL ACTION OF MICROMOLECULAR CONCENTRATIONS OF SUCCINIC ACID, ITS APPLICATION FOR QUANTITATIVE EVALUATION OF LEVEL OF ADRENERGETIC REGULATION IN ORGANISM, MEDIUM AND SET FOR METHOD REALISATION

摘要

FIELD: chemistry, biochemistry.;SUBSTANCE: invention relates to field of biochemistry, cytochemistry, physiology and clinical medicine and is intended for quantitative characteristic if energy process in mitochondria - succinic acid oxidation and its signal action. Essence of cytobiochemical method lies in the following: determination of succinate dehydrogenase (SDG) activity by measuring on blood smear of value of reduced nitro blue tetrazolium (NBT) in lymphocytes is carried out in sample complex, using composition of media, with reagent concentrations brought close to their content in cell. On the basis of said determination also claimed is method of quantitative determination of level of adrenegetic regulation in organism. In order to realise claimed method standard set of dry reagents allowing to unify measurements has been created. Said invention considerably increases sensitivity of measurement of SDG activity in lymphocytes in case of change of state in organism induced by adrenergetic regulation.;EFFECT: increasing sensitivity of measurement of SDG activity in lymphocytes in case of change of state in organism induced by adrenergetic regulation.;4 cl, 2 tbl, 11 ex, 11 dwg