A method for highly sensitive detection of the presence of macromolecules into an aqueous medium

摘要

A method for highly sensitive detection of the presence of macromolecules having a molecular weight of at least 1000 g / mol, which are present in an aqueous medium, comprising the steps of:a) introducing of the aqueous medium, wherein the macromolecules are present, in a sample volume,b) the disassembly of the macromolecules in the aqueous medium into fragments, wherein the disassembly by means of an energy input into the sample volume or by means of a treatment of the macromolecules with a chemical agent for the fragmentation of molecules is carried out,c) introduction of the aqueous medium, wherein the dispersed macromolecules in fragments are present, in a measuring volume of 10 fl to 1 μl,d) acting upon the measuring volume with a first electromagnetic wave, the a fixed frequency of 10 khz to 10 mhz, via at least one pair of electrodes, which passes through an insulation layer is arranged separately from the aqueous medium, ande) receiving the impedance,characterized in that the sample volume at the same time serves as a measuring volume.