A method for the production of dendritic cell - similar cells and use of these cells in in - vitro test methods for determining the influence of exogenous substances

摘要

The technical field of the invention, the field of biotechnology.The present invention relates to the manufacturing method of a new type of dendritic cells, referred to as dendritic cell similar cells. The present invention also relates to the use of these cells in vitro in - - a method for determining the influence of a test substance, such as, for example, the sensitising potential. The generation of the dendritic cell-like cells takes place from human monocytes by co-culture with allogenic keratinocytes. The results are dendritic cell similar cells which, with respect to the expression of surface proteins, in particular of cd1a and cd1c, of previously known natural and in - vitro made dendritic cells differentiate. The dendritic cell-like cells are economically interesting since, in the case of their generation of the expensive use of cytokines and the amount of time are considerably reduced.