DETERMINATION OF MELTING TEMPERATURE BY EQUATION-LESS METHOD

摘要

PPROBLEM TO BE SOLVED: To provide a system and method for determining melting temperature for oligonucleotides based on melt curve data. PSOLUTION: Numerical determination of a first differential coefficient of a melt curve data set is made. A baseline is determined for a first derivative value and the baseline is subtracted from the first differential value to produce modified first differential value. A first maximum value of the modified first differential value is determined and the first maximum value represents a melting temperature Tm of a DNA sample. A model function, such as a Gaussian Mixture Model (GMM) function, with parameters determined using a Levenberg-Marquardt (LM) regression process can also be used to find an approximation to the first derivative curve. The maximum values of the numerically determined first differential value are used as initial conditions for parameters of the model function. The determined parameters provide one or more fractional melting temperature values, which can be returned, for example, displayed or otherwise used for further processing. PCOPYRIGHT: (C)2010,JPO&INPIT