METHOD FOR DETECTING GENE VARIATION RELATED TO THROMBOTIC DISEASE BY ANALYZING VON WILLEBRAND FACTOR NICKING ENZYME GENE

摘要

PPROBLEM TO BE SOLVED: To provide a means attaining early diagnosis and judgement of a morbidity hazard rate of thrombotic disease [such as thrombotic thrombocytopenic purpura (TTP), coronary arterial disease (CAD), and cerebral appoplexy]. PSOLUTION: The method for detecting gene variation having possibility of causing the thrombotic disease [such as TTP, hemolytic uremic syndrome (HUS), CAD, and the cerebral appoplexy] comprises detecting single nucleotide mutation of a von Willebrand factor nicking enzyme gene expressed by 3066539A (expressed by a position in a genomic base sequence of the gene described in GenBank accession number NT_035014.3 and a base after the mutation) and 1193T; 1460G; 1786G; 1992A; 2160A; 2296G; 2724C; 3050A; 3152T (expressed by positions in a cDNA base sequence of the gene described in GenBank accession number NM_139025 and bases after the mutation). A method for testing the morbidity hazard rate of the disease utilizes the method for detecting the gene variation. Further, a gene having the variation, a polynucleotide used in the method, and a reagent kit used in the same are provided. PCOPYRIGHT: (C)2010,JPO&INPIT