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Method for identifying genes involved in memory formation using small interfering RNA (siRNA)

机译:使用小干扰RNA(siRNA)鉴定参与记忆形成的基因的方法

摘要

The invention process can be administered to an animal small interfering RNA sufficiently specific for (a) gene (siRNA), to inhibit the function of the gene; transcription of normal animals not treated, and (b) the animal step of training under conditions sufficient to induce dependent memory formation; including the step of determining the level of transcription dependent memory formation induced by the training of animals and are (c) the processing and transfer in animals on the method of identifying genes or gene products associated with dependent memory formation. To identify genes and gene products, there is provided a use small interfering RNA in hippocampus (siRNA), the invention, temporal and long-term memory context rather than (STM) short-term memory inhibition of genes and gene products I will provide a method of affecting (LTM).
机译:可以将本发明的方法给予对(a)基因(siRNA)足够特异性以抑制基因功能的动物小干扰RNA。未治疗的正常动物的转录,以及(b)在足以诱导依赖性记忆形成的条件下进行的动物训练步骤;包括确定由训练动物引起的转录依赖性记忆形成水平的步骤,以及(c)在鉴定与依赖性记忆形成相关的基因或基因产物的方法中在动物中的加工和转移。为了鉴定基因和基因产物,提供了一种在海马中使用的小干扰RNA(siRNA),本发明提供了对时间和长期记忆的上下文,而不是(STM)对基因和基因产物的短期记忆抑制。影响方法(LTM)。

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