Sensor reading system and grid-type sensor to combine the fluorescence amplification function and joint detection function of label-free

摘要

A grating-based biosensor comprising a substrate having a periodic surface grating structure, wherein the periodic grating structure is constructed in a manner designed for optical interrogation of the sensor with excitation light at at least two discrete wavelengths and polarizations in an evanescent resonance detection mode to thereby produce a luminescence response from a sample placed on the biosensor at at least two discrete emission wavelengths.