METHOD FOR EXTRACTING GENOME DNA FROM SPORE-FORMING BACTERIAL SPORE

摘要

PROBLEM TO BE SOLVED: To provide a method for efficiently extracting and acquiring a genome DNA under a mild condition through extraction and acquisition of genome DNA from spores without carrying out preparation of nutritive cells by germination.;SOLUTION: The spores of spore-forming bacteria are incubated in the presence of a bacteriostatic emulsifying agent in a solution to directly elute and extract a genome from spores without carrying out preparation of nutritive cells by germination of spores. A bacteriostatic surfactant such as a sucrose fatty acid ester, a glycerol fatty acid ester, a polyglycerol fatty acid ester, a sugar alcohol fatty acid ester etc., may be cited as a bacteriostatic surfactant to be used in a method for extracting and acquiring genome DNA from spores. Since the method does not use a means such as a means for grinding spores, a genome is directly extracted by a simple method without causing degeneration such as fractionation of genome DNA.;COPYRIGHT: (C)2010,JPO&INPIT