METHOD FOR ANALYZING ALLERGIC SUBSTANCE IN FOOD

摘要

PROBLEM TO BE SOLVED: To provide a sensitive immunological analysis method and an analysis kit for safely and simply detecting an allergic substance without using a poison such as 2-mercaptoethanol regardless of an altered/native state of the allergic substance (an allergen) in foods, and also provide an aqueous solution for extracting the allergic substance used for them, and a method for extracting the allergic substance.;SOLUTION: The aqueous solution for extracting the allergic substance from the foods contains an alkyl sulfate, a urea and a cysteine, or the alkyl sulfate, the urea, the cysteine and a dithiothreitol. In the aqueous solution, a molar ratio of the contained alkyl sulfate, the urea and the cysteine is 1.0:8.0-250:0.01-100:0-1.0. The allergic substance such as an ovalbumin, a αs1-casein, a β-lactoglobulin, a wheat gliadin, a buckwheat 24kDa protein and an Ara h1 is extracted from the foods, detected by a sandwich ELISA method or an immunochromatographic method, and quantitated.;COPYRIGHT: (C)2010,JPO&INPIT