When detection modulo of the mutation of CYP2D6 and the nuclear acid probe and kit null

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting a mutation at the 100th position (C100T mutation) of a cDNA of a CYP2D6 gene.;SOLUTION: A nucleic acid probe obtained by amplifying a region containing the C100T mutation according to a PCR (polymerase chain reaction), labeling the terminus with a fluorescent dye, reducing the fluorescence of the fluorescent dye when hybridized and having a specific base sequence is used to carry out a melting curve analysis by measuring the fluorescence of the fluorescent dye. The mutation is detected on the basis of the results of the melting curve analysis.;COPYRIGHT: (C)2005,JPO&NCIPI