When detection modulo of the mutation of NAT2*7 and the nuclear acid probe and kit null

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting the 857-position mutation (G857A mutation) of the cDNA of N-acetyltransferase 2 (NAT2) gene.;SOLUTION: The method for detecting the G857A mutation comprises amplifying a zone containing the G857A mutation by PCR (polymerase chain reaction), measuring the fluorescence of a fluorescent pigment with a nucleic acid probe which has a terminal labeled with the fluorescent pigment, reduces the fluorescence of the fluorescent pigment when hybridized, and has a specific base sequence, thereby performing a melting curve analysis, and detecting the mutation on the basis of the result in the melting curve analysis.;COPYRIGHT: (C)2006,JPO&NCIPI