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Method for measuring vitamin D3 receptor binding activity using fluorescence polarization method

机译:荧光偏振法测定维生素D3受体结合活性的方法

摘要

A binding activity can be detected by measuring a change in the degree of fluorescence polarization of a fluorescent-labeled low-molecular weight compound showing a difference between the degree of fluorescence polarization at binding to a receptor and the degree of fluorescence polarization at not binding to the receptor. By using this method, a method of screening a compound binding to a vitamin D3 receptor is provided.
机译:可以通过测量荧光标记的低分子量化合物的荧光偏振度的变化来检测结合活性,该荧光标记的低分子量化合物显示出与受体结合时的荧光偏振度与不与受体结合时的荧光偏振度之间的差异。受体。通过使用该方法,提供了筛选与维生素D3受体结合的化合物的方法。

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