CAPILLARY SIEVING ELECTROPHORESIS WITH A CATIONIC SURFACTANT FOR SIZE SEPARATION OF PROTEINS

摘要

Disclosed herein is a method for size separation of proteins by capillary sieving electrophoresis with cationic surfactant, suitable for molecular-weight determination of proteins in the range between about 14,000 and 500,000, further a composition of a separation medium and of a denaturing solution for said method. In a preferred embodiment, the separation medium comprises a buffer having pH between about 3 and 5.5, a neutral hydrophilic sieving polymer, and between about 0.5 and 30 g/L cationic surfactant.