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CAPILLARY SIEVING ELECTROPHORESIS WITH A CATIONIC SURFACTANT FOR SIZE SEPARATION OF PROTEINS
CAPILLARY SIEVING ELECTROPHORESIS WITH A CATIONIC SURFACTANT FOR SIZE SEPARATION OF PROTEINS
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机译:阳离子表面活性剂毛细管电泳用于蛋白质分离
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摘要
Disclosed herein is a method for size separation of proteins by capillary sieving electrophoresis with cationic surfactant, suitable for size separation of proteins with molecular weights in the range between about 14,000 and about 500,000, further a composition of a separation medium and denaturing solution for said method of separation method. In a preferred embodiment, the separation medium comprises a buffer having pH between about 3 and 5.5, a neutral hydrophilic sieving polymer, and between about 0.5 and about 30 g/L cationic surfactant.
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