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METHODS FOR INCREASING GERMINATION FREQUENCY AND/OR VIGOR BY COLD SHOCK TREATMENT OF CONIFER SOMATIC EMBRYOS DURING DEVELOPMENT
METHODS FOR INCREASING GERMINATION FREQUENCY AND/OR VIGOR BY COLD SHOCK TREATMENT OF CONIFER SOMATIC EMBRYOS DURING DEVELOPMENT
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机译:在发育过程中通过冷休克处理体细胞胚来提高发芽频率和/或活力的方法
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摘要
In one aspect, a method is provided for increasing germination vigor and/or frequency of conifer somatic embryos produced in vitro. The method comprises (a) incubating a plurality of immature conifer somatic embryos for a first incubation period in, or on, a first development media at a temperature in the range of 20° C. to 30° C.; (b) exposing the plurality of immature conifer somatic embryos incubated in accordance with step (a) to a cold temperature in the range of 0° C. to 10° C. for a time period of at least one week; and (c) incubating the plurality of immature conifer somatic embryos treated in accordance with step (b) for a second incubation period in, or on, a second development medium at a temperature in the range of 20° C. to 30° C.
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