Methods for increasing germination vigor by early singulation of conifer somatic embryos

摘要

In one aspect, a method is provided for increasing germination vigor of conifersomatic embryos produced in vitro. The method comprises (a) singulating a pluralityof individual immature conifer somatic embryos from a first culture of embryosafter a first incubation period in a first development media; and (b) contactingthe plurality of singulated immature conifer somatic embryos with a second developmentmedia for a second incubation period.