Optimization of Germinated Conditions for Somatic Embryos in Liriodendron Hybrids

摘要

The development of somatic embryos is a key technology in plant breeding.To gain the best cultural conditions,the factors influencing on germination and transplanting survival rate of the somatic embryos in Liriodendron hybrids were optimized,including the development period of somatic embryos,macro-element,Vitamin C,genotypes,plant hormones and transplant medium in this study.The results showed that the optimal development phase of germination was torpedo-shaped embryo,and the base cultural medium of 3/4 MS with vitamin C can keep normal form from somatic embryos.The germination rate of somatic embryo had obvious genotype difference,among which the hybrid 1×5002 was the most sensitive(82.46%),and the hybrid 1×4088 was the most insensitive(13.24%).The medium with 0.1 mg/L of KT+0.1 mg/L of IBA can help promote the germination of somatic embryogenesis.In addition,the yellow soil was more suitable than mixed peat soil for transplanting Liriodendron hybrids seedlings.Therefore,the obtainment of the optimal conditions has important guidance for production practices of Liriodendron hybrids.