This invention discloses using SPR technology to simultaneously and quantitatively detect the presence of serum markers in pregnant women for the purpose of screening for congenital birth defects. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of representative antibodies used to detect the respective serum markers in pregnant women for the purpose of maternal serum screening for congenital birth defects.
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