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Method for the dissociation of the extracellular haemoglobin molecule of Arenicola marina and the characterization of the protein chains forming the molecule and the nucleotide sequences coding for said protein chains
Method for the dissociation of the extracellular haemoglobin molecule of Arenicola marina and the characterization of the protein chains forming the molecule and the nucleotide sequences coding for said protein chains
Method of dissociating the extracellular haemoglobin molecule of annelids, e.g. Arenicola marina, which can be used to obtain the protein chains forming the molecule. The method includes a step of bringing an extracellular haemoglobin sample from annelids, e.g. Arenicola marina, into contact with at least one dissociating agent, e.g. a mixture containing dithiothreitol (DTT) or tris(2-carboxyethyl)phosphine (TCEP) hydrochloride or beta-mercaptoethanol and a dissociation buffer for a sufficient length of time in order to separate the protein chains from one another.
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