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Method for measuring protease activity of transglutaminase and transglutaminase composition

机译:测定转谷氨酰胺酶的蛋白酶活性的方法和转谷氨酰胺酶组合物

摘要

The invention provides a method for measuring the protease activity of a transglutaminase-containing product comprising: mixing an aqueous solution (sample solution) of a transglutaminase-containing product and an aqueous solution of dimethylcasein in a prescribed ratio of transglutaminase activity to quantity of dimethylcasein, allowing the mixture to stand under prescribed conditions to cause a degradation reaction by protease to progress, adding an acid, filtering, and measuring the concentration of the protein in the filtrate. The invention is particularly useful for transglutaminase formulation for binding application and transglutaminase formulation for Surimi product.
机译:本发明提供了一种用于测量含有转谷氨酰胺酶的产物的蛋白酶活性的方法,该方法包括:将含有转谷氨酰胺酶的产物的水溶液(样品溶液)与二甲基酪蛋白的水溶液以转谷氨酰胺酶活性与二甲基酪蛋白的量的规定比例混合,使混合物在规定的条件下静置以引起蛋白酶降解反应的进行,加入酸,过滤并测量滤液中蛋白质的浓度。本发明对于用于结合应用的转谷氨酰胺酶制剂和用于Surimi产品的转谷氨酰胺酶制剂特别有用。

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