Composition for Separating Sperm from a semen Sample and Methods for Separating Sperm from a semen sample from a non-human animal

摘要

Compositions and Methods useful for processing animal semen for use in Assisted Reproduction.A composition for Separating sperm in a semen Sample which comprises at least a Salt of an alkali metal and / or an alkali earth metal, EDTA, a shock absorber zwitterrionico, particles of silica coated with SILANE and Water, wherein Said composition has a pH of 7.0 - 7.35 and u The osmolarity of 300 - 345 mOsm.Furthermore, a method for preparing Sperm from a semen sample from a non-human animal comprising the step of separating the Sperm of the other constituents of semen by centrifugation through a single layer of a Colloidal formulation. Preferably, the Colloidal composition formulation is mentioned.A method for Separating a subpopulation of Sperm of interest of a semen sample from a non-human animal comprising the steps of providing a Density gradient which comprises at least two layers of the compositionWhere each Layer has a different Density; separate subpopulations of sperm in the semen sample by centrifugation through a Density gradient of sperm; and select the subpopulation of interest.Finally provide Sperm prepared with the methods of the present and the use of the sperm in Artificial Insemination, in vitro fertilization or intracytoplasmic sperm injection.