COMPOSITION FOR SEPARATION OF SPERMATOZOES FROM A SEMEN SAMPLE, METHODS FOR PREPARING SPERMATOZOIDS FROM A NON-HUMAN ANIMAL SEMEN SAMPLE, AND TO SEPARATE A SPERMATOZOID SUBPOPULATION

摘要

A composition for separating sperm from a semen sample, methods for preparing sperm from a semen sample from a non-human animal, and for separating a sperm subpopulation of interest from a semen sample from a non-human animal. It relates to the field of veterinary medicine, more specifically to compositions and methods useful for processing animal semen for use in assisted reproduction. The invention relates to a composition for sperm separation from a semen sample comprising at least one alkali metal and / or alkaline earth metal salt, edta, a zuiterionic buffer, silane-coated silica particles and water. said composition having a pH of 7.0-7.35 and an osmolarity of 300-345 mosm. furthermore, it relates to a method for preparing sperm from a semen sample from a nonhuman animal, comprising the step of separating sperm from other semen constituents by centrifugation through a single layer of a colloid formulation. preferably, the colloid formulation is a composition according to the invention. The invention also relates to a method for separating a sperm subpopulation of interest from a semen sample from a non-human animal, comprising the steps of providing a density gradient comprising at least two layers of a composition of the invention, each layer having a different density; separate the sperm subpopulations in the semen sample by centrifugation through the density gradient; and select the subpopulation of sperm of interest. Finally, the invention relates to sperm prepared with the methods of the invention and to the use of such sperm in artificial insemination, in vitro fertilization or intracytoplasmic sperm injection.