Method for producing sterile Male flowers and parthenocarpic Fruit through Gene Silencing, Associated sequences and vectors containing

摘要

Method for producing sterile Male flowers and parthenocarpic Fruit through Gene Silencing that consists of the following steps: a) obtain the coding sequence of genes homologous to pistillata (PI) of the desired species; (b) to analyse the expression of the sequence obtained for verific Ar is expressed according to the pattern described for Genes pistillata; (c) Analyse complementation mutateTes for the PI PI Gene with the sequence obtained from the desired species to verify that the obtained Gene Sequence has the function of PI; d) manufacturing construction for Gene Silencing an area containing the coding sequence of Pi in a Plant expression vector; (e) incor Built in poracion vector Agrobacterium tumefaciens; (f) transforming plants with interestAgrobacterium tumefaciens vector Silencing and modified by the selection of transformed plants; (g) to verify the absence of contamination by Agrobacterium and corroboration of the transgenic plants by Amplification of the Transgene.Also disclosed vvpi genes of Vitis vinifera Variety Cabernet Sauvignon and Lepi of Lycopersicon esculentum; and the use of these genes to produce sterile Male flowers and fruits without Seeds or parthenocarpic. Jointly disseminated Silencing vectors containing these sequences or part of them.