Process for producing ester nucleosu00ecdeo 5 '- phosphate, acid phosphatase mutant, isolated gene encoding phosphatase mutant.

摘要

A method for producing nucleoside-5'-phosphate ester involves phosphorylating nucleoside biochemically.;Nucleoside-5'-phosphate ester is produced by allowing an acid phosphatase, especially an acid phosphatase having an increased affinity for a nucleoside and/or an increased temperature stability, to act at pH 3.0 to 5.5 on a nucleoside and a phosphate group donor. The phosphate group donor may be selected from polyphosphoric acid or a salt thereof, phenylphosphoric acid or a salt thereof, and carbamyl phosphate or a salt thereof. The resulting nucleoside-5'-phosphate ester may subsequently be collected. As an alternative to using isolated acid phosphatase to catalyse the reaction, a microorganism transformed with a gene encoding a protein having acid phosphatase activity may be employed.