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Procedure for obtaining Synthetic Seed of Sugar Cane (Saccharum spp.)

机译:获得甘蔗(Saccharum spp。)合成种子的程序

摘要

Procedure for the in vitro propagation of the Sugar Cane (Saccharum sp.Synthetic SEED) by using somatic embryogenesis, which manages to overcome the described so far, which introduces important changes in the key steps of the process are: the use of segments of leaves and immature inflorescences as source Field Direct EXPLANT,The establishment of the EXPLANT directly heterogeneous Cell suspensions in a liquid medium in agitation, maturation and germination of somatic embryos in liquid medium from abscisic acid and Osmotic and encapsulation and conversion of embryos in co Ndiciones ex vitro with employment of 2-bromo-5 - (2-bromo-2-nitrovinyl) - Furan in the Capsules.
机译:利用体细胞胚发生法进行甘蔗(Saccharum sp.Synthetic SEED)体外繁殖的方法,到目前为止,该方法设法克服了上述情况,该方法在该方法的关键步骤中引入了重要的变化:使用叶段和未成熟的花序作为直接Field EXPLANT的源,在液体培养基中通过脱落酸和渗透作用搅动,成熟和萌发体细胞胚,在液体培养基中建立EXPLANT直接异质细胞悬浮液,并在体外将胚包封和转化在胶囊中使用2-溴5-(2-溴-2-硝基乙烯基)-呋喃。

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