Method of investigating dynamics of volume changes of physiologically, particularly photo synthetically active samples and apparatus for making the same

摘要

The volume change dynamics of photo synthetically active samples, such as higher or lower plants, algae, photosynthetic bacteria, have been examined by means of a photo acoustic method so far, which does not enable dynamically accurate measurement of transversal changes of these samples in real time in vivo. According to the present invention the transversal change ({DELTAh}) of the position of a measured sample (1) surface exposed to the influence of visible radiation (23), for example actinic light, is measured interferometrically by means of a reflecting target (7), which is in mechanical contact with the measured sample (1) surface, and reflects a measuring beam (5), generated together with a reference beam (6) by a laser source (3). The transversal change ({DELTAh}) of the position of the measured sample (1) surface is then detected from the phase difference of harmonic waves in the beam of interfering radiation (9). The device according to the invention comprises among others a special detection connection of photodetectors (12, 14, 31), a measuring unit (33) and a base module (35) with a computer (16), the design of reflecting targets (7) for liquid and solid samples (1), an optomechanical dimension transmitter (19) and a holder (30) of solid samples (1).