BUTYRYLCHOLINESTERASE AS A MARKER OF LOW-GRADE SYSTEMIC INFLAMMATION

摘要

The invention generally relates to a strategy or method of detecting and diagnosing low-grade systemic inflammatory conditions such as insulin resistance, type 2 diabetes mellitus, hypertension, hyperlipidemias, and Alzheimer's disease using plasma and/or tissue levels of acetylcholinesterase and butyrylcholinesterase as a marker. In addition, for the prevention, treatment, and monitor prognosis of these diseases and other disorders caused by uncontrolled angiogenic activity such as proliferative diabetic retinopathy; and other eye disorders such as macular degeneration; and central nervous system disorders such as Alzheimer's disease; which are characterized by low-grade systemic inflammation, development of a reliable, unique and specific marker is essential. The present invention relates to a strategy or method of detecting, diagnosing, and prognosticating all the above-mentioned conditions using plasma and/or tissue levels of acetylcholinesterase and butyrylcholinesterase as a marker. The method employed to detect acetylcholinesterase and butyrylcholinesterase could include various methods such as but not limited to turbimetric test, or ELISA (enzyme linked immunosorbent assay), fluorometric,. radioimmunoassay, and spectrophotometric methods. The test system proposed can be used in an automated fashion for high throughput detection of many samples, or can be used as a single test or multiple tests as the case may be. The test system can be used daily, weekly, or monthly or at some other appropriate time of interval to detect the presence, progress or regression of the various systemic, local, and/or low-grade systemic inflammatory conditions.