EXPRESSION OF RECOMBINANT PROTEINS IN PLASTIDS OF HIGHER PLANTS AND THE SECRETION OF PROTEIN INTO CYTOPLASM OF THE HOST CELL

摘要

The present invention relates to a method of expressing a foreign protein in the plastid of a host cell and secreting said protein into the cytoplasm of the host cell comprising the steps of making a construct of vector linked to a coding sequence of the fusion protein comprising of signal peptide sequence followed by in-frame fusion to a foreign gene; and stably integrating said construct into the plastid genome. The present invention also relates to a method of targeting the expressed and secreted proteins from the plastids to the nucleus of the host cell. The present invention further relates to the method where the host cell is of any higher plant or any organism including single cell algae.