Method for the characterization and / or identification of substances active mechanisms antimicrobial test.

摘要

Process for identifying or characterizing the active mechanism of an antimicrobial substance, comprising the following steps: a) Set reference spectra by treatment of certain microbial cultures with test substances, the active mechanism is known, and recording, at least one spectrum group of IR, IR, Raman and FT-Raman. b) respective selection at least a range of wavelength of the same or similar structure, to differentiate classes pertaining to the corresponding active mechanism, and classification of the reference spectra into the classes in the database reference also , the reference spectra assigned to a class exhibit identical or similar structure in the range of selected wavelength, which differs significantly from the structure of the reference spectra of other classes in the range selected wavelength c ) treatment of a microbial culture with the substance to be tested d) recording of at least one spectrum (test spectrum) from the group of IR, FT-IR, Raman and FT-Raman; e) Comparison of the spectra of test d) with one or more reference spectra in the database reference, f) Allocation of the test spectra to one, two or more classes of reference spectra in the bank reference data and identification or characterization of the active mechanism.