NEW BACILLUS THURINGIENSIS STRAINS AND THEIR INSECTICIDAL PROTEINS

摘要

Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMGunder accession nos. LMG P-12592,LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins duringsporulation that are toxic to Lepidoptera,more particularly against Noctuidae such as Spodoptera spp. and Agrotisipsilon, against Pyralidae such as Ostrinia nubilalis,and against Yponomeutidae such as Plutella xylostella, and that are encoded bya novel gene. The crystal proteins containpro-toxins, which can yield a toxin as trypsin-digestion product. A plant, thegenome of which is transformed with a DNA sequencethat comes from either one of the strains and that encodes its respectivetoxin, is resistant to Lepidoptera. Each strain, itself, or itscrystals, crystal proteins, protoxin or toxin can be used as the activeingredient in an insecticidal composition for combattingLep-idoptera.