SINGLE-CHAIN POLYPEPTIDES COMPRISING TROPONIN I N-TERMINAL FRAGMENTS AND TROPONIN C

摘要

This invention relates to single-chain polypeptides and their geneticsequences comprising a human cardiac troponin I N-terminal fragment andtroponin C. The N-terminal fragment may or may not have the intact N-terminus.The single-chain polypeptide may be expressed recombinantly, and a linker orspacer polypeptide or peptide may be interposed between the troponinsequences. The single-chain polypeptides have utility as controls orcalibrators for troponin assays, for the purification of troponin subunits andas an antigen for the preparation of antibodies.