FED-BATCH FERMENTATION PROCESS AND CULTURE MEDIUM FOR THE PRODUCTION OF PLASMID DNA IN E. COLI ON A MANUFACTURING SCALE

摘要

A process for producing plasmid DNA E. coli cells comprises a pre-culture andfed batch process. The culture media of the batch phase and the culture mediumadded during the feeding phase are chemically defined. The culture medium ofthe feeding phase contains a growth-limiting substrate and is added, for atleast a fraction of the feeding phase, at a feeding rate that follows a pre-defined exponential function, thereby controlling the specific growth rate ata pre-defined value. The process results in high yield and homogeneity ofplasmid DNA.