IDENTIFICATION OF IMMUNOGLOBULIN (IG) DISORDERS USING FOURIER TRANSFORM INFRARED SPECTROSCOPY

摘要

A method and a corresponding system for obtaining a serum mid-infraredspectroscopic profile using Fourier-transform infrared spectroscopy (FTIR) aredescribed.The method comprises acquiring FTIR spectra for dried sera and preprocessingthe FTIRspectra of sera by differentiation and smoothing to enhance weak spectralfeatures and toremove baseline variations. The preprocessed FTIR spectra are normalized to acommon intensity range, the normalization being performed in a spectral sub-regiondefined by strongest infrared (IR) absorption for a protein to obtain theserumspectroscopic profile. The serum spectroscopic profiles provide a basis todiagnoseimmunoglobulin disorders or to quantify serum immunoglobulin levels.