首页>
外国专利>
METHOD FOR DETECTING LOW CONCENTRATIONS OF A TARGET BACTERIUM THAT USES PHAGES TO INFECT TARGET BACTERIAL CELLS
METHOD FOR DETECTING LOW CONCENTRATIONS OF A TARGET BACTERIUM THAT USES PHAGES TO INFECT TARGET BACTERIAL CELLS
展开▼
机译:检测噬菌体的低浓度的噬菌体感染噬菌体细胞的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The invention is directed to a method for detecting low concentrations of bacteria in liquid solution that may or may not be complex liquid solutions. In one embodiment, immunomagnetic separation (IMS) is used to separate target bacterium that may be in a liquid mixture from other constituents in the mixture. A low concentration of a bacteriophage for the target bacteria is subsequently used to infect target bacterial cells that have been captured using the IMS technique. If at least a certain concentration of target bacterium are present, the bacteriophage will multiply to a point that is detectable. Matrix assisted laser desorption ionization/time-of-flight-mass spectrometry (MALDI/TOF-MS) is then used to produce a mass spectrum that is analyzed to determine if one or more proteins associated with the bacteriophage are present, thereby indirectly indicating that target bacterium were present in the liquid mixture.
展开▼