DETECTION, VISUALIZATION, AND QUANTIFICATION OF MICROVASCULATURE USING CONFOCAL MICROSCOPY

摘要

When detecting and/or imaging microvasculature, images slices of a stained tissue sample are generated using a confocal microscope (12). The image slices are aggregated into vertical stacks, or z-stacks. A thresholding or ray casting technique is applied to the z-stacks to segment out stained microvessels (64). A 3D image representation of the z-stacks with segmented microvessels (64) is rendered for viewing.