INFECTIOUS GENOTYPE 1a, 1b, 2a, 2b, 3a, 5a, 6a and 7a HEPATITIS C VIRUS LACKING THE HYPERVARIABLE REGION 1 (HVR1)

摘要

The present inventors used the previously developed H77/JFH 1T27OOC,A4O8OT (la/2a), J4/JFH 1T2996C,A4827T,ΔHVRI (lb/2a), J6/JFH 1ΔHVRI (2a/2a), J8/JFH 1ΔHVRI (2b/2a), S52/JFH lT27i8G,τ7i6oc (3a/2a), SA13/JFH 1C34O5G,A3696G (5a/2a) and HK6a/JFH lT1389c,A1590G (6a/2a) constructs for the deletion of Hypervariable Region 1 (HVRl) to construct viable, JFH l (genotype 2a) based, genomes. The present inventors serially passaged the viruses in cell culture obtaining relatively high HCV RNA titers and infectivity titers. Sequence analysis of the viruses identified mutations adapting H77/JFH 1T27OOC,A4O8OT,ΔHVR1 (la/2a), J8/JFH 1ΔHVR1 (2b/2a), S52/JFH 1T2718G,T716OC,ΔHVR1 (3a/2a) and J4/JFH 1T2996C,A4827T,ΔHVR1 (lb/2a) to the HVRl deletion.