Infectious genotype 1a, 1b, 2a, 2b, 3a, 5a, 6a and 7a hepatitis C virus lacking the hypervariable region 1 (HVR1)

摘要

The present inventors used the previously developed H77/JFH 1_{T27OOC,A4O8OT} (1a/2a), J4/JFH _{1T2996C,A4827T,ΔHVRI} (1b/2a), J6/JFH _1ΔHVRI (2a/2a), J8/JFH 1_ΔHVRI (2b/2a), S52/JFH 1_{T27i8G,τ7i6oc} (3a/2a), SA13/JFH 1_{C34O5G,A3696G} (5a/2a) and HK6a/JFH 1T_1389c,A1590G (6a/2a) constructs for the deletion of Hypervariable Region 1 (HVR1) to construct viable, JFH 1 (genotype 2a) based, genomes. The present inventors serially passaged the viruses in cell culture obtaining relatively high HCV RNA titers and infectivity titers. Sequence analysis of the viruses identified mutations adapting H77/JFH 1_{T27OOC,A4O8OT,ΔHVR1} (1a/2a), J8/JFH _1ΔHVR1 (2b/2a), S52/JFH 1_{T2718G,T716OC,ΔHVR1} (3a/2a) and J4/JFH 1_{T2996C,A4827T,ΔHVR1} (1b/2a) to the HVR1 deletion.