A METHOD FOR PRODUCING ETHYL (S)-4-CHLORO-3-HYDROXYBUTANOATE BY USE OF CARBONYL REDUCTASE

摘要

A method for preparing ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE) through asymmetric reduction from ethyl 4-chloroacetoacetate (COBE), in which the catalyst is carbonyl reductase having amino acid sequence shown in SEQ ID NO: 2 from Pichia stipitis, the cofactor is NADPH, the conversion ratio for the substrate is more than 95%, and the optical purity of product is 100% enantiomeric excess.