ALTERED PHIC31 INTEGRASES HAVING IMPROVED EFFICIENCY AND SPECIFICITY AND METHODS OF USING SAME

摘要

The present invention relates to the identification, isolation, cloning, expression, purification, and methods of use of altered fC31 integrases. In one aspect, the present invention is directed to a method of site-specifically integrating a polynucleotide sequence of interest in a genome of a target cell using an altered fC31 integrases of the present invention.