GENETIC TESTING METHOD FOR CANCER BY ANALYSIS OF EXPRESSION OF CANCER-RELATING GENE UTILIZING MONOCYTE CONTAINED IN BLOOD SAMPLE

摘要

Disclosed is a genetic testing method which can predict the risk of occurrence of a cancer cell mass or the efficacy of a therapy with high accuracy in an early stage or after prognosis.Specifically disclosed is a genetic testing method for cancer, which comprises: a step (10) of separating a buffy coat layer from a blood sample by centrifugation; a step (20) of separating a monocyte by density gradient centrifugation; a step (30) of extracting total RNA from the monocyte; a step (40) of synthesizing complementary DNA from the total RNA by reverse transcription reaction; a step (50) of carrying out a real-time-PCR (RT-PCR) analysis for a cancer-relating gene; and a step (60) of carrying out a DNA microarray analysis for a cancer-relating gene by employing a DNA microarray as a nucleic acid microarray.