ISOLATION METHOD FOR INNER CELL MASSES AND METHOD FOR PREPARING EMBRYONIC STEM CELL LINES USING THE SAME

摘要

The present invention relates to an isolation method for inner cell masses and a method for preparing embryonic stem cell lines using the same. More particularly, the present invention relates to an isolation method for inner cell masses by placing a blastocyst, from which a zona pellucida has been removed, on feeder cells and covering the blastocyst with a micro cover glass; and pressurizing the blastocyst by the weight of the micro cover glass for a predetermined amount of time to the isolated inner cell masses. Thus, it is possible to harvest inner cell masses with a significantly higher yield than before. Accordingly, the isolation method can establish and proliferate embryonic stem cell lines effectively.