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Method for isolation of inner cell mass and method of preparation of embryonic stem cell lines using inner cell mass isolated by the same
Method for isolation of inner cell mass and method of preparation of embryonic stem cell lines using inner cell mass isolated by the same
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机译:分离内部细胞团的方法和使用由其分离的内部细胞团制备胚胎干细胞系的方法
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摘要
A method for isolation of an inner cell mass and a method for preparation of embryonic stem cell lines using the inner cell mass isolated by the same. A blastocyst being free from a zona pellucida removed therefrom is placed on a feeder cell, and a micro cover glass is put on the blastocyst to apply pressure caused by a weight of the micro cover glass, to the blastocyst for a desired time, so that the inner cell mass may be obtained with considerably improved yield compared to conventional methods, and therefore, an embryonic stem cell line may be efficiently established and proliferated.
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