Surrogate marker directed cDNA cloning of selectively induced mRNAs

摘要

Provided is a method for cDNA cloning of specifically induced mRNAs from single cells using a novel type of genotype/phenotype coupling. In particular, the method enables the selective cloning of a specific mRNA encoding a novel phenotype from at least one single cell by determining a surrogate marker for the respective phenotype. A preferred embodiment is a method for surrogate marker directed cDNA cloning of an antigen induced mRNA sequence encoding the variable chain of an immunoglobulin, wherein the cDNA is cloned from a selected memory B cell by single cell RT-PCR.